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ssiv rt enzyme  (Thermo Fisher)


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  • 90

    Structured Review

    Thermo Fisher ssiv rt enzyme
    Ssiv Rt Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ssiv+enzyme/bio_rxiv__2024__12__17__628988-391-25-22
    Average 90 stars, based on 1 article reviews
    ssiv rt enzyme - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Generated:

    Article Title: ACoRE: Accurate SARS-CoV-2 genome reconstruction for the characterization of intra-host and inter-host viral diversity in clinical samples and for the evaluation of re-infections
    Article Snippet: RNA (5 or 10 μL) was incubated with 1 μL of 60 μM Random Primer Mix (New England Biolabs, Ipswich, MA, USA) and 1 μL of 10 mM dNTPs (New England Biolabs) at 65 °C for 5 min followed by 1 min on ice. .. Subsequently, 4 μL of 5× SSIV buffer, 1 μL of 100 mM DTT, 1 μL of 40 U/μL RNaseOUT, 1 μL of 200 U/μL SSIV enzyme (Thermo Fisher Scientific) and 6 μL nuclease-free water (total reaction volume = 20 μL) were added and the reaction conducted at 23 °C for 10 min, 52 °C for 10 min and 80 °C for 10 min. Two or three cDNAs were generated from every sample, each of which was amplified 2–3 times using the ARTIC protocol. .. 2.5 or 5 μL cDNA were mixed with 3.7 μL of 10 μM primer pools A and B from the ARTIC nCoV-2019 V3 panel (IDT, Coralville, IA, USA), 12.5 μL Q5 high-fidelity DNA polymerase 2× (New England Biolabs) for each of the primer pools, and nuclease-free water to a final volume of 25 μL.

    Article Title: ACoRE: Accurate SARS-CoV-2 genome reconstruction for the characterization of intra-host and inter-host viral diversity in clinical samples and for the evaluation of re-infections.
    Article Snippet: RNA (5 or 10 μL) was incubated with 1 μL of 60 μM Random Primer Mix (New England Biolabs, Ipswich, MA, USA) and 1 μL of 10 mM dNTPs (New England Biolabs) at 65 ◦C for 5 min followed by 1 min on ice. .. Subsequently, 4 μL of 5× SSIV buffer, 1 μL of 100 mM DTT, 1 μL of 40 U/μL RNaseOUT, 1 μL of 200 U/μL SSIV enzyme (Thermo Fisher Scientific) and 6 μL nuclease-free water (total reaction volume = 20 μL) were added and the reaction conducted at 23 ◦C for 10 min, 52 ◦C for 10 min and 80 ◦C for 10 min. Two or three cDNAs were generated from every sample, each of which was amplified 2–3 times using the ARTIC protocol. .. 2.5 or 5 μL cDNA were mixed with 3.7 μL of 10 μM primer pools A and B from the ARTIC nCoV-2019 V3 panel (IDT, Coralville, IA, USA), 12.5 μL Q5 high-fidelity DNA polymerase 2× (New England Biolabs) for each of the primer pools, and nuclease-free water to a final volume of 25 μL.

    Amplification:

    Article Title: ACoRE: Accurate SARS-CoV-2 genome reconstruction for the characterization of intra-host and inter-host viral diversity in clinical samples and for the evaluation of re-infections
    Article Snippet: RNA (5 or 10 μL) was incubated with 1 μL of 60 μM Random Primer Mix (New England Biolabs, Ipswich, MA, USA) and 1 μL of 10 mM dNTPs (New England Biolabs) at 65 °C for 5 min followed by 1 min on ice. .. Subsequently, 4 μL of 5× SSIV buffer, 1 μL of 100 mM DTT, 1 μL of 40 U/μL RNaseOUT, 1 μL of 200 U/μL SSIV enzyme (Thermo Fisher Scientific) and 6 μL nuclease-free water (total reaction volume = 20 μL) were added and the reaction conducted at 23 °C for 10 min, 52 °C for 10 min and 80 °C for 10 min. Two or three cDNAs were generated from every sample, each of which was amplified 2–3 times using the ARTIC protocol. .. 2.5 or 5 μL cDNA were mixed with 3.7 μL of 10 μM primer pools A and B from the ARTIC nCoV-2019 V3 panel (IDT, Coralville, IA, USA), 12.5 μL Q5 high-fidelity DNA polymerase 2× (New England Biolabs) for each of the primer pools, and nuclease-free water to a final volume of 25 μL.

    Article Title: ACoRE: Accurate SARS-CoV-2 genome reconstruction for the characterization of intra-host and inter-host viral diversity in clinical samples and for the evaluation of re-infections.
    Article Snippet: RNA (5 or 10 μL) was incubated with 1 μL of 60 μM Random Primer Mix (New England Biolabs, Ipswich, MA, USA) and 1 μL of 10 mM dNTPs (New England Biolabs) at 65 ◦C for 5 min followed by 1 min on ice. .. Subsequently, 4 μL of 5× SSIV buffer, 1 μL of 100 mM DTT, 1 μL of 40 U/μL RNaseOUT, 1 μL of 200 U/μL SSIV enzyme (Thermo Fisher Scientific) and 6 μL nuclease-free water (total reaction volume = 20 μL) were added and the reaction conducted at 23 ◦C for 10 min, 52 ◦C for 10 min and 80 ◦C for 10 min. Two or three cDNAs were generated from every sample, each of which was amplified 2–3 times using the ARTIC protocol. .. 2.5 or 5 μL cDNA were mixed with 3.7 μL of 10 μM primer pools A and B from the ARTIC nCoV-2019 V3 panel (IDT, Coralville, IA, USA), 12.5 μL Q5 high-fidelity DNA polymerase 2× (New England Biolabs) for each of the primer pools, and nuclease-free water to a final volume of 25 μL.



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